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  • Dear Partners and Friends in our ocean and coastal community,

    Oceans provide vital resources and services for sustaining humankind including food, recreation, transportation, energy, nutrient cycling and climate moderation, and they substantially contribute to our economy. However, the chemistry of the oceans is changing in ways that will have impacts on these services and resources far into the future.

    Recognizing the need for a comprehensive interagency plan to address the increasing impacts of ocean acidification, Congress passed the Federal Ocean Acidification Research and Monitoring Act of 2009 (FOARAM Act), which defines ocean acidification as “the decrease in pH of the Earth’s oceans and changes in ocean chemistry caused by chemical inputs from the atmosphere, including carbon dioxide.” Coastal and estuarine acidification, to the extent that the cause of the acidification can be traced back to anthropogenic atmospheric inputs to the ocean, are assumed to be covered by this Strategic Plan for Federal Research and Monitoring of Ocean Acidification (Strategic Plan) wherever ocean acidification is referenced.

    The FOARAM Act called for the Subcommittee on Ocean Science and Technology (SOST) to establish an Interagency Working Group on Ocean Acidification (IWG-OA). The Act also explicitly called for developing a strategic research plan to guide “Federal research and monitoring on ocean acidification that will provide for an assessment of the impacts of ocean acidification on marine organisms and marine ecosystems and the development of adaption and mitigation strategies to conserve marine organisms and marine ecosystems.” Per requirements of the FOARAM, the original draft plan was open for public comment for two months and also was reviewed by the National Research Council. Edits to this plan were made to address comments that were received. Details about editing decisions are available upon request.

    The IWG-OA was chartered in October 2009. These agencies have come together to provide a thoughtful, strategic approach to understand and address the rapidly emerging problem of ocean acidification. This plan is essential to guide federal ocean acidification investments and activities over the next decade and beyond. It will provide a better understanding of the process of ocean acidification, its effects on marine ecosystems, and the steps that must be taken to minimize harm from ocean acidification.

    We organized the plan around the following seven priority areas 1) research, 2) monitoring, 3) modeling, 4) technology development, 5) socioeconomic impacts, 6) education and outreach and 7) data management. This plan is the result of a collaborative, thoughtful, and dedicated effort by a large number of people. My thanks goes out to all who contributed to this plan.

    Sincerely,

    Elizabeth Jewett

    Chair of the IWG-OA

     

    Ned Cyr

    Former Chair of the IWG-OA

    Author(s): Elizabeth Jewett , Ned Cyr, Richard Feely, Kenric Osgood, Christopher Sabine, Krisa Arzayus, Steve Gittings, David Garrison, Jennifer Culbertson, Kenli Kim, Jason Grear, Christopher Moore, Paula Bontempi, Kathy Tedesco, Bret Wolfe, Lisa Robbins, Kimberly Yates, Justine Kim
  • Among the three types of technologies available in the fisheries sector in India, seaweed farming, initially promoted as a livelihood option, has emerged as the one area which probably has the maximum potential for up-scaling. This paper has examined the structure, conduct and performance of the value chain in seaweed farming in India inquiring into the production, institutional, marketing, social and community relationships in small-scale seaweed farming in the Ramanathapuram district of Tamil Nadu and the concept of self-help groups (SHG) as an increasingly workable option for coastal resources management. The value chain analysis of the sector has substantially proved that committed and synergistic production, marketing and institutional arrangements enabled by corporate leadership, offers considerable savings in transaction costs. The SHG model has also shown strong gender orientation in the initial years of seaweed culture in the district contributing to strong structural foundations to the movement. The seaweed sector in the coastal India has all the potential to rise from the low-income conditions normally associated with basic livelihood activities to higher levels of employment-income-consumption relationships.

    Author(s): Krishnan, M, Narayanakumar, R
  • Studies were made on agar from 8 red algae and algin from 10 brown algae collected from Tuticorin, Manapad, Tiruchendur, ldinthakarai and Kovalam along south Tamil Nadu Coast. The yield of agar varied from 18.0 to 48.3% and gel strength from 9 to 117 gm/sq. cm. The algin content ranged from 8.7 to 34.0%. These agar and algin yielding seaweeds can be exploited from the above places and used as additional source of raw material for the production of agar and sodium alginate.

    Author(s): Kaliaperumal, N, Kalimuthu, S, Ramalingam, J R
  • Studies on the growth and reproductive behaviour of Gracilaria corticata carried out at Mandapam and Pudumadam in the Gulf of Mannar, over a period of three years from 1968 to 1970 have been presented, along with some observations on the distribution and harvest of the crop. The growth of the species appears to be irregular and the peak growth occurs during June to August/September and December to February/March, Seasonal changes have not been observed in the abundance of reproductive plants which occurred throughout the year. Tetrasporic plants have predominant in the populations examined at Mandapam and Pudumadam than the sexual plants. Harvesting experiment conducted at Pudumadam showed that the time of harvest and periodic collection of plants influence the rate of production and density of the alga in the natural habitats and that it is profitable to harvest G. corticata twice in a year, during June to August/September and December to February/March.

    Author(s): Rao, M Umamaheswara
  • Suto (1950 a, 1950 b) studied the relation between sporulation of seaweeds and seawater temperature. He observed that shedding of tetraspores Started in Gelidium when the seawater temperature rose to 2 0 ' ~ and carpospores to 2 4 ) ~ I Further he observed that there was an optimum temperature range for shedding of spores and abnormal temperature delayed or hastened shedding by about 20 days. Also, he noticed that shedding of monospores of Gelidium amansii increased during calm weather and decreased during rough weather. Segawa et. a1.(1955 a.1955 b)and Jones (1957) studied the nature and mechanism of carpospore liberation in Gracilaria verrucosa (Huds) papenfs. Takeuchi et. a1.(1956) studied the daily output of monospores from cultures of the Conchocelis phase of Porphyra tenera Kjellm. Oza and Krishnamunhy (1968) investigated the carposporic rhythm in Gracilaria verrucosa and reported a peak sporulation of the alga in December and a gradual decline during March - May. In this paper observations on the nature of carpospore output in Gracilaria cortlcata J. Ag. and an estimate of the quantity of spores liberated by the alga during August to December 1969 are presented and discussed.

    Author(s): Mohan Joseph, M, Krishnamurthy, V
  • Colloidal solutions of silver nanoparticles (AgNPs) were synthesized by gamma Co-60 irradiation using different stabilizers, namely polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), alginate, and sericin. The particle size measured from TEM images was 4.3, 6.1, 7.6, and 10.2 nm for AgNPs/PVP, AgNPs/PVA, AgNPs/alginate, and AgNPs/sericin, respectively. The influence of different stabilizers on the antibacterial activity of AgNPs was investigated. Results showed that AgNPs/alginate exhibited the highest antibacterial activity against Escherichia coli (E. coli) among the as-synthesized AgNPs. Handwash solution has been prepared using Na lauryl sulfate as surfactant, hydroxyethyl cellulose as binder, and 15 mg/L of AgNPs/alginate as antimicrobial agent. The obtained results on the antibacterial test of handwash for the dilution to 3 mg AgNPs/L showed that the antibacterial efficiency against E. coli was of 74.6%, 89.8%, and 99.0% for the contacted time of 1, 3, and 5 min, respectively. Thus, due to the biocompatibility of alginate extracted from seaweed and highly antimicrobial activity of AgNPs synthesized by gamma Co-60 irradiation, AgNPs/alginate is promising to use as an antimicrobial agent in biomedicine, cosmetic, and in other fields.

    Author(s): Nguyen Quoc Hien, Le Quang Luan, Bui Duy Du, Nguyen Thi Kim Lan, Nguyen Ngoc Duy, Le Anh Quoc, Dang Van Phu
  • Vegetated coastal habitats have been identified as important carbon sinks. In contrast to angiosperm-based habitats such as seagrass meadows, salt marshes and mangroves, marine macroalgae have largely been excluded from discussions of marine carbon sinks. Macroalgae are the dominant primary producers in the coastal zone, but they typically do not grow in habitats that are considered to accumulate large stocks of organic carbon. However, the presence of macroalgal carbon in the deep sea and sediments, where it is effectively sequestered from the atmosphere, has been reported. A synthesis of these data suggests that macroalgae could represent an important source of the carbon sequestered in marine sediments and the deep ocean. We propose two main modes for the transport of macroalgae to the deep ocean and sediments: macroalgal material drifting through submarine canyons, and the sinking of negatively buoyant macroalgal detritus. A rough estimate suggests that macroalgae could sequester about 173 TgC yr−1 (with a range of 61–268 TgC yr−1) globally. About 90% of this sequestration occurs through export to the deep sea, and the rest through burial in coastal sediments. This estimate exceeds that for carbon sequestered in angiosperm-based coastal habitats.

    Author(s): Carlos M. Duarte , Dorte Krause-Jensen
  • The Department of Energy’s Bioenergy Technology Office (BETO) collaborates with a wide range of institutions towards the development and deployment of biofuels and bioproducts. To facilitate this effort, BETO and its partner national laboratories develop detailed techno-economic assessments (TEA) of biofuel production technologies as part of the development of design cases and state of technology (SOT) analyses. A design case is a TEA that outlines a target case for a particular biofuel pathway. It enables preliminary identification of data gaps and research and development needs and provides goals and targets against which technology progress is assessed. On the other hand, an SOT analysis assesses progress within and across relevant technology areas based on actual experimental results relative to technical targets and cost goals from design cases and includes technical, economic, and environmental criteria as available.

    BETO also develops supply chain sustainability analyses (SCSA) for key biofuel production technologies that are the subject of design case or SOT analyses (Dunn et al. 2013). The SCSA utilizes a life-cycle analysis to estimate the energy use and greenhouse gas (GHG) emissions associated with biofuel production and assists in comparing several biofuel pathways. This report documents an SCSA of whole algae hydrothermal liquefaction (AHTL) as the conversion technology to produce renewable diesel (RD). Jones et al. (2014) developed the design case process model that provides the material and energy intensity of the feedstock conversion step in the SCSA.

    The SCSA production stages for microalgae-derived RD are presented in Figure 1. Various inputs (red boxes) can be considered for each supply chain step (green boxes). These inputs can include energy, fertilizers for biomass growth, and any materials that may be needed during the conversion process. The major environmental output from the system is GHG emissions, which come from direct sources like fuel combustion during a processing step or indirect sources like fertilizer production. Another common output is coproducts, which can be used to displace materials or energy from other production processes. There can be difficulties in allocating emissions to these co-products (Wang et al., 2011), so care is needed during their consideration.

    The SCSA for RD produced via AHTL starts with feedstock production, which requires nutrients (fertilizers), water (not considered in this study), and energy in the form of electricity and other fuels, e.g., natural gas. After production, the feedstock is transported to the conversion facility, or biorefinery, using energy in the form of a transportation fuel. In the case of microalgae, cultivation ponds are assumed to be co-located with the conversion facility (Davis et al., 2012; Frank et al., 2011) meaning a transportation fuel is not required. However, energy is needed for pumping the biomass from the harvesting units to the biorefinery. For the algae-to-RD production reported here, the harvested feedstock goes to a thermal conversion process, which includes material inputs like catalysts and sulfuric acid. A small amount of naphtha, which was treated as a liquid fuel, is produced along with RD in the AHTL pathway. No other co-products are produced in the fully integrated AHTL algae-to- RD pathway. The total supply chain emissions burdens were allocated to total fuel produced, including naphtha and RD.

    The renewable fuel, after the conversion process, is transported to a fueling station by train, barge, and truck. The biogenic CO2 released when the fuel is combusted balance out with the atmospheric CO2 that the algae incorporated when it was growing (Frank et al., 2011). The emissions described above are the so-called, “fuel cycle” emissions. Emissions are also associated with the construction of the plant (Canter et al., 2014). These “infrastructure cycle” emissions were estimated in this study.

    Author(s): Christopher M. Kinchin, Ryan Davis, Lesley Snowden-Swan, Yunhua Zhu, Sue Jones, Edward D. Frank, Jennifer B. Dunn, Ambica Koushik Pegallapati
  • Seaweeds or macroalgae are attractive candidates for carbon capture, while also supplying a sustainable photosynthetic bioenergy feedstock, thanks to their cultivation potential in offshore marine farms. Seaweed cultivation requires minimal external nutrient requirements and allows for year-round production of biomass. Despite this potential, there remain significant challenges associated with realizing large-scale, sustainable agronomics, as well as in the development of an efficient biomass deconstruction and conversion platform to fuels and products. Recent biotechnology progress in the identification of enzymatic de- construction pathways, tailored to complex polymers in seaweeds, opens up op- portunities for more complete utilization of seaweed biomass components. Effective, scalable, and economically viable conversion processes tailored to seaweed are discussed and gaps are identified for yield and efficiency improvements.

    Author(s): Lieve M.L. Laurens, Madeline Lane, Robert S. Nelson
  • The effects of Tasco®, a product made from the brown seaweed (Ascophyllum nodosum) were tested for the ability to protect Caenorhabditis elegans against Pseudomonas aeruginosa infection. A water extract of Tasco® (TWE) reduced P. aeruginosa inflicted mortality in the nematode. The TWE, at a concentration of 300 µg/mL, offered the maximum protection and induced the expression of innate immune response genes viz.; zk6.7 (Lypases), lys-1 (Lysozyme), spp-1 (Saponin like protein), f28d1.3 (Thaumatin like protein), t20g5.7 (Matridin SK domain protein), abf-1 (Antibacterial protein) and f38a1.5 (Lectin family protein). Further, TWE treatment also affected a number of virulence components of the P. aeuroginosa and reduced its secreted virulence factors such as lipase, proteases and toxic metabolites; hydrogen cyanide and pyocyanin. Decreased virulence factors were associated with a significant reduction in expression of regulatory genes involved in quorum sensing, lasI, lasR, rhlI and rhlR. In conclusion, the TWE-treatment protected the C. elegans against P. aeruginosa infection by a combination of effects on the innate immunity of the worms and direct effects on the bacterial quorum sensing and virulence factors.

    Author(s): Balakrishnan Prithivira, Alan T. Critchley, Franklin Evans, Wajahatullah Khan, Saveetha Kandasamy

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